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Spec-seq: determining protein–DNA-binding specificity by sequencing

The specificity of protein–DNA interactions can be determined directly by sequencing the bound and unbound fractions in a standard binding reaction. The procedure is easy and inexpensive, and the accuracy can be high for thousands of sequences assayed in parallel. From the measurements, simple model...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Brief Funct Genomics
Main Authors: Stormo, Gary D., Zuo, Zheng, Chang, Yiming Kenny
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4366588/
https://ncbi.nlm.nih.gov/pubmed/25362070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/bfgp/elu043
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