shRNA Off-Target Effects In Vivo: Impaired Endogenous siRNA Expression and Spermatogenic Defects

RNA interference (RNAi) is widely used to determine the function of genes. We chose this approach to assess the collective function of the highly related reproductive homeobox 3 (Rhox3) gene paralogs. Using a Rhox3 short hairpin (sh) RNA with 100% complementarity to all 8 Rhox3 paralogs, expressed f...

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Bibliographic Details
Published in:PLoS One
Main Authors: Song, Hye-Won, Bettegowda, Anilkumar, Oliver, Daniel, Yan, Wei, Phan, Mimi H., de Rooij, Dirk G., Corbett, Mark A., Wilkinson, Miles F.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2015
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4366048/
https://ncbi.nlm.nih.gov/pubmed/25790000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0118549
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