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Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity
Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR loci to generate immunological memory. The Escherichia coli Cas1–Cas2 complex mediates spacer acquisition in vivo, but the molecular mechanism of this process is unknown. Here we show that the purified Cas1–Cas2 comp...
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| Publicado no: | Nature |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4359072/ https://ncbi.nlm.nih.gov/pubmed/25707795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature14237 |
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