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Quantification of Mature MicroRNAs Using Pincer Probes and Real-Time PCR Amplification

The robust and reliable detection of small microRNAs (miRNAs) is important to understand the functional significance of miRNAs. Several methods can be used to quantify miRNAs. Selectively quantifying mature miRNAs among miRNA precursors, pri-miRNAs, and other miRNA-like sequences is challenging beca...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Huang, Tinghua, Yang, Jun, Liu, Guopin, Jin, Wei, Liu, Zhi, Zhao, Shuhong, Yao, Min
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4359002/
https://ncbi.nlm.nih.gov/pubmed/25768430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0120160
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