Real-time quantification of microRNAs by stem–loop RT–PCR

A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related mi...

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Dades bibliogràfiques
Autors principals: Chen, Caifu, Ridzon, Dana A., Broomer, Adam J., Zhou, Zhaohui, Lee, Danny H., Nguyen, Julie T., Barbisin, Maura, Xu, Nan Lan, Mahuvakar, Vikram R., Andersen, Mark R., Lao, Kai Qin, Livak, Kenneth J., Guegler, Karl J.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2005
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1292995/
https://ncbi.nlm.nih.gov/pubmed/16314309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni178
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