Real-time quantification of microRNAs by stem–loop RT–PCR

A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related mi...

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Dettagli Bibliografici
Autori principali: Chen, Caifu, Ridzon, Dana A., Broomer, Adam J., Zhou, Zhaohui, Lee, Danny H., Nguyen, Julie T., Barbisin, Maura, Xu, Nan Lan, Mahuvakar, Vikram R., Andersen, Mark R., Lao, Kai Qin, Livak, Kenneth J., Guegler, Karl J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2005
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1292995/
https://ncbi.nlm.nih.gov/pubmed/16314309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni178
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