Real-time quantification of microRNAs by stem–loop RT–PCR

A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related mi...

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Detalles Bibliográficos
Main Authors: Chen, Caifu, Ridzon, Dana A., Broomer, Adam J., Zhou, Zhaohui, Lee, Danny H., Nguyen, Julie T., Barbisin, Maura, Xu, Nan Lan, Mahuvakar, Vikram R., Andersen, Mark R., Lao, Kai Qin, Livak, Kenneth J., Guegler, Karl J.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2005
Assuntos:
Methods Online
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1292995/
https://ncbi.nlm.nih.gov/pubmed/16314309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni178
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