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A new mathematical model for relative quantification in real-time RT–PCR

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as...

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Detalhes bibliográficos
Autor principal: Pfaffl, Michael W.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC55695/
https://ncbi.nlm.nih.gov/pubmed/11328886
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