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Improved quantitative real-time RT–PCR for expression profiling of individual cells
The real-time quantitative polymerase chain reaction (rtqPCR) has overcome the limitations of conventional, time-consuming quantitative PCR strategies and is maturing into a routine tool to quantify gene expression levels, following reverse transcription (RT) of mRNA into complementary DNA (cDNA). E...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2002
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC137434/ https://ncbi.nlm.nih.gov/pubmed/12202777 |
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