Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

BACKGROUND: Probe based detection assays form the mainstay of transcript quantification. Problems with these assays include varying hybridization efficiencies of the probes used for transcript quantification and the expense involved. We examined the ability of a standardized competitive RT-PCR (StaR...

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Publié dans:	Mol Cancer
Auteurs principaux:	Pagliarulo, Vincenzo, George, Ben, Beil, Stephen J, Groshen, Susan, Laird, Peter W, Cai, Jie, Willey, James, Cote, Richard J, Datar, Ram H
Format:	Artigo
Langue:	Inglês
Publié:	BMC 2004
Sujets:	Research
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344741/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14741054/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1476-4598-3-5
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