Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

BACKGROUND: Probe based detection assays form the mainstay of transcript quantification. Problems with these assays include varying hybridization efficiencies of the probes used for transcript quantification and the expense involved. We examined the ability of a standardized competitive RT-PCR (StaR...

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Detalhes bibliográficos
Main Authors: Pagliarulo, Vincenzo, George, Ben, Beil, Stephen J, Groshen, Susan, Laird, Peter W, Cai, Jie, Willey, James, Cote, Richard J, Datar, Ram H
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2004
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC344741/
https://ncbi.nlm.nih.gov/pubmed/14741054
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1476-4598-3-5
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