Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

BACKGROUND: Probe based detection assays form the mainstay of transcript quantification. Problems with these assays include varying hybridization efficiencies of the probes used for transcript quantification and the expense involved. We examined the ability of a standardized competitive RT-PCR (StaR...

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Detalles Bibliográficos
Publicado en:Mol Cancer
Main Authors: Pagliarulo, Vincenzo, George, Ben, Beil, Stephen J, Groshen, Susan, Laird, Peter W, Cai, Jie, Willey, James, Cote, Richard J, Datar, Ram H
Formato: Artigo
Idioma:Inglês
Publicado: BMC 2004
Assuntos:
Research
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344741/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14741054/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1476-4598-3-5
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