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Real-time PCR quantification of precursor and mature microRNA
microRNAs (miRNAs) are challenging molecules to amplify by PCR because the miRNA precursor consists of a stable hairpin and the mature miRNA is roughly the size of a standard PCR primer. Despite these difficulties, successful real-time RT-PCR technologies have been developed to amplify and quantify...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2663046/ https://ncbi.nlm.nih.gov/pubmed/18158130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2007.09.006 |
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