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Real-time PCR quantification of precursor and mature microRNA

microRNAs (miRNAs) are challenging molecules to amplify by PCR because the miRNA precursor consists of a stable hairpin and the mature miRNA is roughly the size of a standard PCR primer. Despite these difficulties, successful real-time RT-PCR technologies have been developed to amplify and quantify...

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Dettagli Bibliografici
Autori principali: Schmittgen, Thomas D., Lee, Eun Joo, Jiang, Jinmai, Sarkar, Anasuya, Yang, Liuqing, Elton, Terry S., Chen, Caifu
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663046/
https://ncbi.nlm.nih.gov/pubmed/18158130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2007.09.006
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