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A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets
There are relatively few protocols described for the in situ detection of microRNA (miRNA) and they often use cryostat sections, signal amplification and hybridization or washes of 50−60 °C. This protocol describes in situ miRNA detection that can be done in paraffin-embedded, formalin-fixed tissue....
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| Main Authors: | , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2009
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2709217/ https://ncbi.nlm.nih.gov/pubmed/19131963 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.215 |
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