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A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets

There are relatively few protocols described for the in situ detection of microRNA (miRNA) and they often use cryostat sections, signal amplification and hybridization or washes of 50−60 °C. This protocol describes in situ miRNA detection that can be done in paraffin-embedded, formalin-fixed tissue....

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Библиографические подробности
Главные авторы: Nuovo, Gerard J, Elton, Terry S, Nana-Sinkam, Patrick, Volinia, Stefano, Croce, Carlo M, Schmittgen, Thomas D
Формат: Artigo
Язык:Inglês
Опубликовано: 2009
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2709217/
https://ncbi.nlm.nih.gov/pubmed/19131963
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.215
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