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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Probe-based quantitative PCR (qPCR) is a favoured method for measuring transcript abundance, since it is one of the most sensitive detection methods that provides an accurate and reproducible analysis. Probe-based chemistry offers the least background fluorescence as compared to other (dye-based) ch...
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| Publicado en: | J Vis Exp |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
MyJove Corporation
2015
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4541571/ https://ncbi.nlm.nih.gov/pubmed/25938938 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52586 |
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