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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Probe-based quantitative PCR (qPCR) is a favoured method for measuring transcript abundance, since it is one of the most sensitive detection methods that provides an accurate and reproducible analysis. Probe-based chemistry offers the least background fluorescence as compared to other (dye-based) ch...
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| Udgivet i: | J Vis Exp |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4541571/ https://ncbi.nlm.nih.gov/pubmed/25938938 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52586 |
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