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Quantification of Mature MicroRNAs Using Pincer Probes and Real-Time PCR Amplification

The robust and reliable detection of small microRNAs (miRNAs) is important to understand the functional significance of miRNAs. Several methods can be used to quantify miRNAs. Selectively quantifying mature miRNAs among miRNA precursors, pri-miRNAs, and other miRNA-like sequences is challenging beca...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Huang, Tinghua, Yang, Jun, Liu, Guopin, Jin, Wei, Liu, Zhi, Zhao, Shuhong, Yao, Min
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4359002/
https://ncbi.nlm.nih.gov/pubmed/25768430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0120160
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