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Application of meta- and para- phenylenediamine as enhanced oxime ligation catalysts for protein labeling, PEGylation, immobilization and release

Meta- and para- phenylenediamines have recently been shown to catalyze oxime and hydrazone ligation reactions at rates much faster than aniline, a commonly used catalyst. Here, it is demonstrated how these new catalysts can be used in a generally applicable procedure for fluorescent labeling, PEGyla...

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Detalhes bibliográficos
Publicado no:Curr Protoc Protein Sci
Main Authors: Mahmoodi, Mohammad M., Rashidian, Mohammad, Zhang, Yi, Distefano, Mark D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4357315/
https://ncbi.nlm.nih.gov/pubmed/25640893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps1504s79
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