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Selective Labeling of Polypeptides Using Protein Farnesyltransferase via Rapid Oxime Ligation
An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protei...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3495172/ https://ncbi.nlm.nih.gov/pubmed/20967387 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc03305g |
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