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Selective Labeling of Polypeptides Using Protein Farnesyltransferase via Rapid Oxime Ligation

An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protei...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Rashidian, Mohammad, Dozier, Jonathan K., Lenevich, Stepan, Distefano, Mark D.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2010
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3495172/
https://ncbi.nlm.nih.gov/pubmed/20967387
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc03305g
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