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Ligand-Detected Relaxation Dispersion NMR Spectroscopy: Dynamics of preQ(1)–RNA Binding

An NMR-based approach to characterizing the binding kinetics of ligand molecules to biomolecules, like RNA or proteins, by ligand-detected Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments is described. A (15)N-modified preQ(1) ligand is used to acquire relaxation dispersion experim...

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Publicat a:Angew Chem Int Ed Engl
Autors principals: Moschen, Thomas, Wunderlich, Christoph Hermann, Spitzer, Romana, Levic, Jasmin, Micura, Ronald, Tollinger, Martin, Kreutz, Christoph
Format: Artigo
Idioma:Inglês
Publicat: 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4353840/
https://ncbi.nlm.nih.gov/pubmed/25403518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201409779
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