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Characterizing Protein Modifications by Reactive Metabolites using Magnetic Bead Bioreactors and LC-MS/MS

We report here label-free metabolite-protein adduct detection and identification employing magnetic beads coated with metabolic enzymes as bioreactors to generate metabolites and possible metabolite-protein adducts for analysis by liquid chromatography-tandem mass spectrometry.

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Bibliographische Detailangaben
Veröffentlicht in:Chem Commun (Camb)
Hauptverfasser: Li, Dandan, Fu, You-Jun, Rusling, James F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4348200/
https://ncbi.nlm.nih.gov/pubmed/25693065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5cc00420a
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