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Live cell imaging of cytosolic NADH-NAD(+) redox state using a genetically encoded fluorescent biosensor
NADH is an essential redox cofactor in numerous metabolic reactions, and the cytosolic NADH-NAD(+) redox state is a key parameter in glycolysis. Conventional NADH measurements rely on chemical determination or autofluorescence imaging, which cannot assess NADH specifically in the cytosol of individu...
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| Publicado en: | Methods Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4330558/ https://ncbi.nlm.nih.gov/pubmed/24052382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-622-1_7 |
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