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A genetically encoded fluorescent reporter of ATP/ADP ratio
A fluorescent sensor of adenylate nucleotides was constructed by combining a circularly permuted variant of green fluorescent protein with a bacterial regulatory protein, GlnK1, from Methanococcus jannaschii. The affinity for Mg-ATP is below 100 nM, as seen for the other members of the bacterial PII...
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| Main Authors: | , , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
2009
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2633436/ https://ncbi.nlm.nih.gov/pubmed/19122669 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1288 |
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