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Imaging Intracellular pH in Live Cells with a Genetically-Encoded Red Fluorescent Protein Sensor

Intracellular pH affects protein structure and function, and proton gradients underlie the function of organelles such as lysosomes and mitochondria. We engineered a genetically-encoded pH sensor by mutagenesis of the red fluorescent protein mKeima, providing a new tool to image intracellular pH in...

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Detalhes bibliográficos
Main Authors: Tantama, Mathew, Hung, Yin Pun, Yellen, Gary
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3126897/
https://ncbi.nlm.nih.gov/pubmed/21631110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja202902d
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