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Imaging Intracellular pH in Live Cells with a Genetically-Encoded Red Fluorescent Protein Sensor
Intracellular pH affects protein structure and function, and proton gradients underlie the function of organelles such as lysosomes and mitochondria. We engineered a genetically-encoded pH sensor by mutagenesis of the red fluorescent protein mKeima, providing a new tool to image intracellular pH in...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3126897/ https://ncbi.nlm.nih.gov/pubmed/21631110 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja202902d |
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