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Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation

Small solutes are useful probes of large conformational changes in RNA polymerase (RNAP)-promoter interactions and other biopolymer processes. In general, a large effect of a solute on an equilibrium constant (or rate constant) indicates a large change in water-accessible biopolymer surface area in...

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Publicat a:Methods Mol Biol
Autors principals: Ruff, Emily, Kontur, Wayne S., Record, M. Thomas
Format: Artigo
Idioma:Inglês
Publicat: 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4324510/
https://ncbi.nlm.nih.gov/pubmed/25665568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2392-2_14
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