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One-step DNA melting in the RNA polymerase cleft opens the initiation bubble to form an unstable open complex

Though opening of the start site (+1) region of promoter DNA is required for transcription by RNA polymerase (RNAP), surprisingly little is known about how and when this occurs in the mechanism. Early events at the λP(R) promoter load this region of duplex DNA into the active site cleft of Escherich...

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Detalhes bibliográficos
Main Authors: Gries, Theodore J., Kontur, Wayne S., Capp, Michael W., Saecker, Ruth M., Record, M. Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2890804/
https://ncbi.nlm.nih.gov/pubmed/20483995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1000967107
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