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Solute Probes of Conformational Changes in Open Complex Formation by E. coli RNA Polymerase at the λP(R) Promoter: Evidence for Unmasking of the Active Site in the Isomerization Step and for Large-Scale Coupled Folding in the Subsequent Conversion to RP(o)
Transcription initiation is a multi-step process involving a series of requisite conformational changes in RNA polymerase (R) and promoter DNA (P) that create the open complex (RP(o)). Here we use the small solutes urea and glycine betaine (GB) to probe the extent and type of surface area changes in...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2631401/ https://ncbi.nlm.nih.gov/pubmed/16475805 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi051835v |
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