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Late Steps in the Formation of E. coli RNA Polymerase-λP(R) Promoter Open Complexes: Characterization of Conformational Changes by Rapid [Perturbant] Upshift Experiments

The formation of the transcriptionally-competent open complex (RP(o)) by E. coli RNA polymerase at the λP(R) promoter involves at least three steps and two kinetically-significant intermediates (I(1) and I(2)). Understanding the sequence of conformational changes (rearrangements in the jaws of RNAP,...

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Autors principals: Kontur, Wayne S., Saecker, Ruth M., Capp, Michael W., Record, M. Thomas
Format: Artigo
Idioma:Inglês
Publicat: 2007
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396949/
https://ncbi.nlm.nih.gov/pubmed/18191943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.11.064
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