Fluorescence Resonance Energy Transfer Characterization of DNA Wrapping in Closed and Open Escherichia coli RNA Polymerase–λP(R) Promoter Complexes

Initial recognition of promoter DNA by RNA polymerase (RNAP) is proposed to trigger a series of conformational changes beginning with bending and wrapping of the 40–50 bp of DNA immediately upstream of the −35 region. Kinetic studies demonstrated that the presence of upstream DNA facilitates bending...

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Publicado no:Biochemistry
Main Authors: Sreenivasan, Raashi, Heitkamp, Sara, Chhabra, Munish, Saecker, Ruth, Lingeman, Emily, Poulos, Mikaela, McCaslin, Darrell, Capp, Michael W., Artsimovitch, Irina, Record, M. Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4830751/
https://ncbi.nlm.nih.gov/pubmed/26998673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00125
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