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Fluorescence Resonance Energy Transfer Characterization of DNA Wrapping in Closed and Open Escherichia coli RNA Polymerase–λP(R) Promoter Complexes

Initial recognition of promoter DNA by RNA polymerase (RNAP) is proposed to trigger a series of conformational changes beginning with bending and wrapping of the 40–50 bp of DNA immediately upstream of the −35 region. Kinetic studies demonstrated that the presence of upstream DNA facilitates bending...

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Publicat a:Biochemistry
Autors principals: Sreenivasan, Raashi, Heitkamp, Sara, Chhabra, Munish, Saecker, Ruth, Lingeman, Emily, Poulos, Mikaela, McCaslin, Darrell, Capp, Michael W., Artsimovitch, Irina, Record, M. Thomas
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4830751/
https://ncbi.nlm.nih.gov/pubmed/26998673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00125
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