Fluorescence-Detected Conformational Changes in Duplex DNA in Open Complex Formation by E. coli RNA Polymerase: Upstream Wrapping and Downstream Bending Precede Clamp Opening and Insertion of the Downstream Duplex

FRET (fluorescence resonance energy transfer) between far-upstream(−100) and downstream(+14) cyanine dyes showed extensive bending/wrapping of λP(R) promoter DNA on E. coli RNA polymerase (RNAP) in closed and open complexes (CC, OC). Here we determine the kinetics and mechanism of DNA bending/wrappi...

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Publicado en:Biochemistry
Main Authors: Sreenivasan, Raashi, Shkel, Irina A., Chhabra, Munish, Drennan, Amanda, Heitkamp, Sara, Wang, Hao-Che, Sridevi, Malavika A., Plaskon, Dylan, McNerney, Christina, Callies, Katelyn, Cimperman, Clare K., Record, M. Thomas
Formato: Artigo
Idioma:Inglês
Publicado: 2020
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7269339/
https://ncbi.nlm.nih.gov/pubmed/32216369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.0c00098
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