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Evidence for a tyrosine-adenine stacking interaction and for a short-lived open intermediate subsequent to initial binding of Escherichia coli RNA polymerase to promoter DNA

Bacterial RNA polymerase and a “sigma” transcription factor form an initiation-competent “open” complex at a promoter by disruption of about 14 base pairs. Strand separation is likely initiated at the highly conserved -11 A-T base pair. Amino acids in conserved region 2.3 of the main E. coli sigma f...

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Detalhes bibliográficos
Main Authors: Schroeder, Lisa A., Gries, Theodore J., Saecker, Ruth M., Record, M. Thomas, Harris, Michael E., deHaseth, Pieter L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2677906/
https://ncbi.nlm.nih.gov/pubmed/18976666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.10.023
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