Effect of vector-expressed shRNAs on hTERT expression

AIM: To study the effect of short hairpin RNAs (shRNAs) expressed from DNA vector on hTERT expression. METHODS: Oligonucleotides coding for four shRNAs against hTERT were cloned into a mammalian shRNA expression vector pUC18U6 to form pUC18U6ht1-4, which were then introduced into HepG2 cells by usin...

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Bibliographic Details
Published in:World J Gastroenterol
Main Authors: Guo, Ying, Liu, Jun, Li, Ying-Hui, Song, Tian-Bao, Wu, Jing, Zheng, Cai-Xia, Xue, Cai-Fang
Format: Artigo
Language:Inglês
Published: Baishideng Publishing Group Inc 2005
Subjects:
Basic Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4305658/
https://ncbi.nlm.nih.gov/pubmed/15902727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i19.2912
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