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Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression
shRNA is a powerful tool for inhibiting gene expression. One limitation has been that this technique has been used primarily to target a single gene. This protocol expands upon previous methods by describing a knockdown vector that facilitates cloning of multiple shRNAs; this allows targeted knockdo...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2753455/ https://ncbi.nlm.nih.gov/pubmed/19713955 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.145 |
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