Functional and intracellular localization properties of U6 promoter-expressed siRNAs, shRNAs, and chimeric VA1 shRNAs in mammalian cells

RNA polymerase III (Pol III) expression systems for short hairpin RNAs (U6 shRNAs or chimeric VA1 shRNAs) or individually expressed sense/antisense small interfering RNA (siRNA) strands have been used to trigger RNA interference (RNAi) in mammalian cells. Here we show that individually expressed siR...

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Autors principals: Lee, Nan Sook, Kim, Daniel H., Alluin, Jessica, Robbins, Marjorie, Gu, Shuo, Li, Haitang, Kim, James, Salvaterra, Paul M., Rossi, John J.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2008
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2525953/
https://ncbi.nlm.nih.gov/pubmed/18697923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1014008
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