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Functional and intracellular localization properties of U6 promoter-expressed siRNAs, shRNAs, and chimeric VA1 shRNAs in mammalian cells

RNA polymerase III (Pol III) expression systems for short hairpin RNAs (U6 shRNAs or chimeric VA1 shRNAs) or individually expressed sense/antisense small interfering RNA (siRNA) strands have been used to trigger RNA interference (RNAi) in mammalian cells. Here we show that individually expressed siR...

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Bibliografski detalji
Glavni autori: Lee, Nan Sook, Kim, Daniel H., Alluin, Jessica, Robbins, Marjorie, Gu, Shuo, Li, Haitang, Kim, James, Salvaterra, Paul M., Rossi, John J.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2008
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2525953/
https://ncbi.nlm.nih.gov/pubmed/18697923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1014008
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