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CRISPR-Cas9 Genome Editing of a Single Regulatory Element Nearly Abolishes Target Gene Expression in Mice

OBJECTIVE: To ascertain the importance of a single regulatory element in the control of Cnn1 expression using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) genome editing. APPROACH AND RESULTS: The CRISPR/Cas9 system was used to produce 3/18 foun...

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Detalhes bibliográficos
Publicado no:Arterioscler Thromb Vasc Biol
Main Authors: Han, Yu, Slivano, Orazio J., Christie, Christine K., Cheng, Albert W., Miano, Joseph M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4304932/
https://ncbi.nlm.nih.gov/pubmed/25538209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/ATVBAHA.114.305017
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