Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9

The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR plasmids for single and multiplexed gene editing in U. maydis...

Volledige beschrijving

Bewaard in:
Bibliografische gegevens
Gepubliceerd in:Bio Protoc
Hoofdauteurs: Schuster, Mariana, Trippel, Christine, Happel, Petra, Lanver, Daniel, Reißmann, Stefanie, Kahmann, Regine
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Bio-Protocol 2018
Onderwerpen:
Methods Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8328660/
https://ncbi.nlm.nih.gov/pubmed/34395750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2928
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!

Gelijkaardige items