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Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9
The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR plasmids for single and multiplexed gene editing in U. maydis...
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| Pubblicato in: | Bio Protoc |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Bio-Protocol
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8328660/ https://ncbi.nlm.nih.gov/pubmed/34395750 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2928 |
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