Enzymatic Synthesis of Modified Oligonucleotides by PEAR Using Phusion and KOD DNA Polymerases

Antisense synthetic oligonucleotides have been developed as potential gene-targeted therapeutics. We previously reported polymerase–endonuclease amplification reaction (PEAR) for amplification of natural and 5′-O-(1-thiotriphosphate) (S)-modified oligonucleotides. Here, we extended the PEAR techniqu...

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Bibliographic Details
Published in:Nucleic Acid Ther
Main Authors: Wang, Xuxiang, Zhang, Jianye, Li, Yingjia, Chen, Gang, Wang, Xiaolong
Format: Artigo
Language:Inglês
Published: Mary Ann Liebert, Inc. 2015
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Original Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4296748/
https://ncbi.nlm.nih.gov/pubmed/25517220
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/nat.2014.0513
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