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Enzymatic Synthesis of Modified Oligonucleotides by PEAR Using Phusion and KOD DNA Polymerases
Antisense synthetic oligonucleotides have been developed as potential gene-targeted therapeutics. We previously reported polymerase–endonuclease amplification reaction (PEAR) for amplification of natural and 5′-O-(1-thiotriphosphate) (S)-modified oligonucleotides. Here, we extended the PEAR techniqu...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acid Ther |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Mary Ann Liebert, Inc.
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4296748/ https://ncbi.nlm.nih.gov/pubmed/25517220 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/nat.2014.0513 |
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