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A Rat Primary Hepatocyte Culture Model for Aging Studies
The purpose of this protocol is to establish a primary hepatocyte culture system as a suitable model to examine age-related changes in Phase II detoxication gene expression. Hepatocytes are isolated using a two-step collagenase perfusion technique from young (3 to 6 months) and old (24 to 28 months)...
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| 發表在: | Curr Protoc Toxicol |
|---|---|
| Main Authors: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2008
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4290156/ https://ncbi.nlm.nih.gov/pubmed/23045003 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140856.tx1407s37 |
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