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Maintenance of differentiated rat hepatocytes in primary culture.

Normal adult rat hepatocytes remained viable and functional for at least 43 days when plated on collagen-coated dishes and fed chemically defined medium supplemented with dimethyl sulfoxide (Me2SO). Hepatocytes isolated by collagenase perfusion and cultured in the presence or absence of Me2SO were (...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Isom, H C, Secott, T, Georgoff, I, Woodworth, C, Mummaw, J
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1985
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397753/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3858822/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.10.3252
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