Maintenance of differentiated rat hepatocytes in primary culture.
Normal adult rat hepatocytes remained viable and functional for at least 43 days when plated on collagen-coated dishes and fed chemically defined medium supplemented with dimethyl sulfoxide (Me2SO). Hepatocytes isolated by collagenase perfusion and cultured in the presence or absence of Me2SO were (...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1985
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397753/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3858822/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.10.3252 |
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