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A Rat Primary Hepatocyte Culture Model for Aging Studies

The purpose of this protocol is to establish a primary hepatocyte culture system as a suitable model to examine age-related changes in Phase II detoxication gene expression. Hepatocytes are isolated using a two-step collagenase perfusion technique from young (3 to 6 months) and old (24 to 28 months)...

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Detalhes bibliográficos
Publicado no:Curr Protoc Toxicol
Main Authors: Shenvi, Swapna V., Dixon, Brian M., Shay, Kate Petersen, Hagen, Tory M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4290156/
https://ncbi.nlm.nih.gov/pubmed/23045003
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140856.tx1407s37
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