EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae

Development of strains for efficient production of chemicals and pharmaceuticals requires multiple rounds of genetic engineering. In this study, we describe construction and characterization of EasyClone vector set for baker's yeast Saccharomyces cerevisiae, which enables simultaneous expressio...

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Dettagli Bibliografici
Pubblicato in:FEMS Yeast Res
Autori principali: Jensen, Niels B, Strucko, Tomas, Kildegaard, Kanchana R, David, Florian, Maury, Jérôme, Mortensen, Uffe H, Forster, Jochen, Nielsen, Jens, Borodina, Irina
Natura: Artigo
Lingua:Inglês
Pubblicazione: BlackWell Publishing Ltd 2014
Soggetti:
Research Articles
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282123/
https://ncbi.nlm.nih.gov/pubmed/24151867
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1567-1364.12118
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