EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae

Development of strains for efficient production of chemicals and pharmaceuticals requires multiple rounds of genetic engineering. In this study, we describe construction and characterization of EasyClone vector set for baker's yeast Saccharomyces cerevisiae, which enables simultaneous expressio...

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Enregistré dans:
Détails bibliographiques
Publié dans:FEMS Yeast Res
Auteurs principaux: Jensen, Niels B, Strucko, Tomas, Kildegaard, Kanchana R, David, Florian, Maury, Jérôme, Mortensen, Uffe H, Forster, Jochen, Nielsen, Jens, Borodina, Irina
Format: Artigo
Langue:Inglês
Publié: BlackWell Publishing Ltd 2014
Sujets:
Research Articles
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282123/
https://ncbi.nlm.nih.gov/pubmed/24151867
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1567-1364.12118
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