A teaching protocol demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of Saccharomyces cerevisiae and Yarrowia lipolytica

We present a teaching protocol suitable for demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of industrially relevant yeasts Saccharomyces cerevisiae and Yarrowia lipolytica, using β-carotene production as a case study. The protocol details all steps required to generate...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:FEMS Yeast Res
Prif Awduron: Milne, N, Tramontin, L R R, Borodina, I
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2019
Pynciau:
Protocol
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC8260333/
https://ncbi.nlm.nih.gov/pubmed/31556952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsyr/foz062
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