EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9

Saccharomyces cerevisiae is an established industrial host for production of recombinant proteins, fuels and chemicals. To enable stable integration of multiple marker‐free overexpression cassettes in the genome of S. cerevisiae, we have developed a vector toolkit EasyClone‐MarkerFree. The integrati...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Pubblicato in:Biotechnol J
Autori principali: Jessop‐Fabre, Mathew M, Jakočiūnas, Tadas, Stovicek, Vratislav, Dai, Zongjie, Jensen, Michael K, Keasling, Jay D, Borodina, Irina
Natura: Artigo
Lingua:Inglês
Pubblicazione: WILEYߚVCH Verlag 2016
Soggetti:
Biotech Method
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5094547/
https://ncbi.nlm.nih.gov/pubmed/27166612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/biot.201600147
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi