EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9

Saccharomyces cerevisiae is an established industrial host for production of recombinant proteins, fuels and chemicals. To enable stable integration of multiple marker‐free overexpression cassettes in the genome of S. cerevisiae, we have developed a vector toolkit EasyClone‐MarkerFree. The integrati...

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Bibliografski detalji
Izdano u:Biotechnol J
Glavni autori: Jessop‐Fabre, Mathew M, Jakočiūnas, Tadas, Stovicek, Vratislav, Dai, Zongjie, Jensen, Michael K, Keasling, Jay D, Borodina, Irina
Format: Artigo
Jezik:Inglês
Izdano: WILEYߚVCH Verlag 2016
Teme:
Biotech Method
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5094547/
https://ncbi.nlm.nih.gov/pubmed/27166612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/biot.201600147
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