EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9

Saccharomyces cerevisiae is an established industrial host for production of recombinant proteins, fuels and chemicals. To enable stable integration of multiple marker‐free overexpression cassettes in the genome of S. cerevisiae, we have developed a vector toolkit EasyClone‐MarkerFree. The integrati...

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Detalhes bibliográficos
Publicado no:Biotechnol J
Main Authors: Jessop‐Fabre, Mathew M, Jakočiūnas, Tadas, Stovicek, Vratislav, Dai, Zongjie, Jensen, Michael K, Keasling, Jay D, Borodina, Irina
Formato: Artigo
Idioma:Inglês
Publicado em: WILEYߚVCH Verlag 2016
Assuntos:
Biotech Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5094547/
https://ncbi.nlm.nih.gov/pubmed/27166612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/biot.201600147
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