EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae

Development of strains for efficient production of chemicals and pharmaceuticals requires multiple rounds of genetic engineering. In this study, we describe construction and characterization of EasyClone vector set for baker's yeast Saccharomyces cerevisiae, which enables simultaneous expressio...

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Bibliografiske detaljer
Udgivet i:FEMS Yeast Res
Main Authors: Jensen, Niels B, Strucko, Tomas, Kildegaard, Kanchana R, David, Florian, Maury, Jérôme, Mortensen, Uffe H, Forster, Jochen, Nielsen, Jens, Borodina, Irina
Format: Artigo
Sprog:Inglês
Udgivet: BlackWell Publishing Ltd 2014
Fag:
Research Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282123/
https://ncbi.nlm.nih.gov/pubmed/24151867
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1567-1364.12118
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