Antibody Stabilization of Peptide–MHC Multimers Reveals Functional T Cells Bearing Extremely Low-Affinity TCRs

Fluorochrome-conjugated peptide–MHC (pMHC) multimers are commonly used in combination with flow cytometry for direct ex vivo visualization and characterization of Ag-specific T cells, but these reagents can fail to stain cells when TCR affinity and/or TCR cell-surface density are low. pMHC multimer...

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Detaylı Bibliyografya
Yayımlandı:J Immunol
Asıl Yazarlar: Tungatt, Katie, Bianchi, Valentina, Crowther, Michael D., Powell, Wendy E., Schauenburg, Andrea J., Trimby, Andrew, Donia, Marco, Miles, John J., Holland, Christopher J., Cole, David K., Godkin, Andrew J., Peakman, Mark, Straten, Per Thor, Svane, Inge Marie, Sewell, Andrew K., Dolton, Garry
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: AAI 2015
Konular:
Novel Immunological Methods
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4273996/
https://ncbi.nlm.nih.gov/pubmed/25452566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4049/jimmunol.1401785
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