Antibody Stabilization of Peptide–MHC Multimers Reveals Functional T Cells Bearing Extremely Low-Affinity TCRs

Fluorochrome-conjugated peptide–MHC (pMHC) multimers are commonly used in combination with flow cytometry for direct ex vivo visualization and characterization of Ag-specific T cells, but these reagents can fail to stain cells when TCR affinity and/or TCR cell-surface density are low. pMHC multimer...

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Detalles Bibliográficos
Publicado en:J Immunol
Main Authors: Tungatt, Katie, Bianchi, Valentina, Crowther, Michael D., Powell, Wendy E., Schauenburg, Andrea J., Trimby, Andrew, Donia, Marco, Miles, John J., Holland, Christopher J., Cole, David K., Godkin, Andrew J., Peakman, Mark, Straten, Per Thor, Svane, Inge Marie, Sewell, Andrew K., Dolton, Garry
Formato: Artigo
Idioma:Inglês
Publicado: AAI 2015
Assuntos:
Novel Immunological Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4273996/
https://ncbi.nlm.nih.gov/pubmed/25452566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4049/jimmunol.1401785
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