Antibody Stabilization of Peptide–MHC Multimers Reveals Functional T Cells Bearing Extremely Low-Affinity TCRs

Fluorochrome-conjugated peptide–MHC (pMHC) multimers are commonly used in combination with flow cytometry for direct ex vivo visualization and characterization of Ag-specific T cells, but these reagents can fail to stain cells when TCR affinity and/or TCR cell-surface density are low. pMHC multimer...

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Опубликовано в: :J Immunol
Главные авторы: Tungatt, Katie, Bianchi, Valentina, Crowther, Michael D., Powell, Wendy E., Schauenburg, Andrea J., Trimby, Andrew, Donia, Marco, Miles, John J., Holland, Christopher J., Cole, David K., Godkin, Andrew J., Peakman, Mark, Straten, Per Thor, Svane, Inge Marie, Sewell, Andrew K., Dolton, Garry
Формат: Artigo
Язык:Inglês
Опубликовано: AAI 2015
Предметы:
Novel Immunological Methods
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4273996/
https://ncbi.nlm.nih.gov/pubmed/25452566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4049/jimmunol.1401785
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