Antibody Stabilization of Peptide–MHC Multimers Reveals Functional T Cells Bearing Extremely Low-Affinity TCRs

Fluorochrome-conjugated peptide–MHC (pMHC) multimers are commonly used in combination with flow cytometry for direct ex vivo visualization and characterization of Ag-specific T cells, but these reagents can fail to stain cells when TCR affinity and/or TCR cell-surface density are low. pMHC multimer...

Cijeli opis

Spremljeno u:
Bibliografski detalji
Izdano u:J Immunol
Glavni autori: Tungatt, Katie, Bianchi, Valentina, Crowther, Michael D., Powell, Wendy E., Schauenburg, Andrea J., Trimby, Andrew, Donia, Marco, Miles, John J., Holland, Christopher J., Cole, David K., Godkin, Andrew J., Peakman, Mark, Straten, Per Thor, Svane, Inge Marie, Sewell, Andrew K., Dolton, Garry
Format: Artigo
Jezik:Inglês
Izdano: AAI 2015
Teme:
Novel Immunological Methods
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4273996/
https://ncbi.nlm.nih.gov/pubmed/25452566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4049/jimmunol.1401785
Oznake: Dodaj oznaku
Bez oznaka, Budi prvi tko označuje ovaj zapis!

Similar Items