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Evaluation of Disulfide Bond Position to Enhance the Thermal Stability of a Highly Stable Single Domain Antibody

Single domain antibodies are the small recombinant variable domains derived from camelid heavy-chain-only antibodies. They are renowned for their stability, in large part due to their ability to refold following thermal or chemical denaturation. In addition to refolding after heat denaturation, A3,...

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Veröffentlicht in:PLoS One
Hauptverfasser: Zabetakis, Dan, Olson, Mark A., Anderson, George P., Legler, Patricia M., Goldman, Ellen R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2014
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4272287/
https://ncbi.nlm.nih.gov/pubmed/25526640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0115405
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