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Sequence Tolerance of a Single-Domain Antibody with a High Thermal Stability: Comparison of Computational and Experimental Fitness Profiles

[Image: see text] The sequence fitness of a llama single-domain antibody with an unusually high thermal stability is explored by a combined computational and experimental study. Starting with the X-ray crystallographic structure, RosettaBackrub simulations were applied to model sequence–structure to...

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Publicat a:ACS Omega
Autors principals: Olson, Mark A., Legler, Patricia M., Zabetakis, Daniel, Turner, Kendrick B., Anderson, George P., Goldman, Ellen R.
Format: Artigo
Idioma:Inglês
Publicat: American Chemical Society 2019
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6648363/
https://ncbi.nlm.nih.gov/pubmed/31460140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.9b00730
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